| Woodstock Cycling Club (WCC) | Woodstock | Club |
| Waterloo Cycling Club | Kitchener-Waterloo | Club |
| Walden Mountain Bike Club | Sudbury | Club |
| Velocity Bike Club | Pembroke | Club |
| Turkey Point Mountain Bike Club | Turkey Point | Club |
| The Wild Bettys | Toronto | Club |
| Team Van Go | Dufferin | Club |
| Simcoe County Mountain Bike Club | Simcoe County | Club |
| Shorthills Cycling Club | Port Colborne | Club |
| Rocket Mountain Biking Club | Windsor | Club |
| Pedal Pushers | Northern Bruce Peninsula | Club |
| Ottawa Valley Mountain Bike Association (OVMBA) | Pembroke | Club |
| Ottawa Mountain Bike Association (OMBA) | Ottawa | Club |
| Oakville Cycling Club | Oakville | Club |
| MTB Kingston | Kingston | Club |
| MTB CORNWALL | SDG | Club |
| Mountainview Cycling Club | Midland | Club |
| Mountain Bikers United | Richmond Hill | Club |
| Lapdogs Cycling | Toronto | Club |
| James Bay Cycling Club | Moosonee | Club |
| Hamilton Cycling Club | Hamilton | Club |
| HAFTA - Halton Agreement Forest Trail Association | Milton | Club |
| GORBA PATROL | Guelph | Club |
| Durham Mountain Biking Association | Durham Region | Club |
| Dio Cane Mountain Bike CLub | Burlington | Club |