| Team Van Go | Dufferin | Club |
| Club Minaki | Kenora | Club |
| Dio Cane Mountain Bike CLub | Burlington | Club |
| Caledon Cycling Club | Caledon | Club |
| Waterloo Cycling Club | Kitchener-Waterloo | Club |
| Woodstock Cycling Club (WCC) | Woodstock | Club |
| Defiant MTB | Greater Toronto Area - Southern Ontario | Club |
| Mountain Bikers United | Richmond Hill | Club |
| James Bay Cycling Club | Moosonee | Club |
| Simcoe County Mountain Bike Club | Simcoe County | Club |
| Turkey Point Mountain Bike Club | Turkey Point | Club |
| MTB Kingston | Kingston | Club |
| HAFTA - Halton Agreement Forest Trail Association | Milton | Club |
| The Wild Bettys | Toronto | Club |
| MTB CORNWALL | SDG | Club |
| Bikes and Beers Adventures | Burlington | Club |
| CORC - Collingwood Offroad Cycling Club | Collingwood/ Georgian Bay Are | Club |
| Bikefit Sunflowers | Niagara Region | Club |
| Pedal Pushers | Northern Bruce Peninsula | Club |
| Bloomfield Bicycle Club | Bloomfield | Club |
| Bolton Mountain Bike Club (BMBC) | Bolton | Club |
| Central East Sporting Coalition (CESCO) | Coe Hill | Club |
| GORBA PATROL | Guelph | Club |
| Hamilton Cycling Club | Hamilton | Club |
| Lapdogs Cycling | Toronto | Club |
