| Alleghany Highland Trail Club (AHTC) | Clifton Forge | Club |
| Blue Ridge Cyclery Racing | Charlottesville | Club |
| Charlottesville Bicycle Club (CAMBC) | Charlottesville | Club |
| Culpeper Area Mountain Biking Organization (CAMBO) | Culpeper | Club |
| Eastern Virginia Mountain Bike Association (EVMA) | | Club |
| FAMBE | Fredericksburg | Club |
| Fredericksburg Area Trail Management & User Group (FATMUG) | Fredericksburg | Club |
| Fredericksburg Trails Alliance | Fredericksburg | Club |
| Greater Lynchburg Off-Road Cyclists | Lynchburg | Club |
| Hampton Roads Mountain Bikers | Hampton Roads | Club |
| Lonesome Pine Cycling | Big Stone Gap | Club |
| Mid-Atlantic Off-Road Enthusiasts (MORE) | Fairfax | Club |
| MTB LoCo (Mountain Bike Loudoun County | Loudoun | Club |
| New River Valley Bicycle Association (NRVBA) | Blacksburg | Club |
| Patrick County Mountain Bike Club | Bassett | Club |
| Potomac Velo Club (PVC) | Northern Virginia | Race Team |
| Queen City Cycling Club | Staunton | Club |
| Roanoke IMBA (RIMBA) | Roanoke | Club |
| rvaMORE | Richmond area | Club |
| Shenandoah Valley Bicycle Coalition (SVBC) | Harrisonburg | Club |
| Southside Trailblazers | Southwest VA | Club |
| SVMBA | Danville | Club |
| VeloPigs | | Club |
| Winchester Wheelmen | Winchester | Club |
