| Cranky Chicks Cycling | Greensboro | Club |
| Roadies & Dirties Cycling Club | Davidson County | Club |
| Friends of Greensboro Parks and Recreation Foundation (FGPRF) | Greensboro | Club |
| Friends of DuPont Forest (DUPONT) | Brevard | Club |
| Fort Bragg Mountain Bike Riders | Fort Bragg/Fayetteville | Club |
| Kernersville Cycling Club | Kernersville | Club |
| Triad Mountain Bike Group (MeetUp)**defunct** | Triad (Winston-Salem/Greensboro/High Point) | Club |
| Sea Level Cycling | Eastern North Carolina | Club |
| Down East Cyclists | Jacksonville, NC | Club |
| http://kernersvillecyclingclub.com/ | Kernersville, NC | Club |
| Northwest North Carolina Mountain Bike Alliance | Northwest North Carolina | Club |
| East Carolina velo Cycling club | Greenville | Club |
| Charlotte Area Cycling | Charlotte | Club |
| Triangle Off-road Cyclists (TORC) | Raleigh / Durham / Chapel Hill | Club |
| Asheville BOMB | Asheville | Club |
| Charlotte BOMB | Charlotte | Club |
| Piedmont Area Singletrack Alliance (PASA) | Charlotte | Club |
| Nantahala Area SORBA | Nantahala | Club |
| Dirt Divas | Charlotte | Club |
| Catawba Valley Heritage Alliance (CVHA) | Newton | Club |
| Boone Area Cyclists | Boone | Club |
| Brushy Mountain Cycling Club (BMCC) | North Wilkesboro | Club |
| Alamance TrailBlazers | Burlington | Club |
| Cape Fear SORBA | Cape Fear | Club |
| Southern Appalachian Bicycle Association | Western NC | Club |
