| Wilderness Trekkers | Orlando | Club |
| Virginia Key Bicycle Club | Miami | Club |
| Tallahassee Mountain Bike Association | Tallahassee | Club |
| Swamp Mountain Bike Club SWAMP | Saint Petersberg | Club |
| Suwannee Bicycle Association (SBA) | Gainesville | Club |
| SORBA Orlando | Orlando | Club |
| SORBA Jax | Jacksonville | Club |
| Sarasota County Offroad Riders (SCORR) | Sarasota County | Club |
| Ridge Riders Mountain Bike Association (RRMBA) | Lakeland | Club |
| Pensacola Off Road Cyclists (PORC) | Pensacola | Club |
| Ocala Mountain Bike Association (OMBA) | Ocala / Santos | Club |
| MTB Chicks | South Florida | Club |
| Miami Hawks MTB Club | miami | Club |
| Markham Park Trail Building Fund (MPTBF) | Sunrise | Club |
| Graham Swamp Trail Crew Inc. | Flagler County | Club |
| Gainesville Cycling Club | Gainesville | Club |
| Florida BOMB | | Club |
| Florida Bicycle Association | Longwood | Club |
| Flagler Area Biking SORBA | Palm Coast | Club |
| Fat of the Land | Tallahassee | Club |
| Dirt Dogs of Florida | Miami | Club |
| Club Tread | South Florida | Club |
| Club Scrub | Treasure Coast - Palm Beach, Martin & St Lucie Counties | Club |
| Club Mud | Ft. Lauderdale Area | Club |
| Citrus Park Mountain Bike Club | Tampa/Citrus Park/Westchase | Club |
