| Alcoa Mountain Bike Association | Morristown | Club |
| Appalachian Mountain Bike Club (AMBC) | Knoxville | Club |
| Big South Fork Bicycle Club | Oneida | Club |
| CAMBA TN | Clarksville | Club |
| Chattanooga Area Singletrack Alliance (CASA) | Chattanooga | Club |
| Chattanooga Bicycle Club | Chattanooga | Club |
| Clinch Valley Trail Alliance | Oak Ridge | Club |
| COGS Mountain Bike Club | Ft. Campbell, KY/ Clarksville, TN | Club |
| Cycling Club at ETSU | Johnson City | Club |
| Five Rivers Mountain Bike Club | Morristown | Club |
| Five Rivers Mountain Bike Club - SORBA | East Tenn | Club |
| Highland Rimmers Bicycle Club | Tullahoma | Club |
| Mid-South Trails Association | Memphis | Club |
| SORBA Middle Tennessee | Nashville | Club |
| Southern Off-Road Bicycle Association Tri-Cities Chapter (SORBA) | Northeast Tennessee | Club |
| Stanky Creek Cycling | Memphis | Race Team |
| Sugar Camp Hollow International Mountain Bike Association (SCHIMBA) | Savannah | Club |
| Velo Vixens Cycling Club | Chattanooga | Club |
